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KMID : 0545120120220081170
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 8 p.1170 ~ p.1176
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α-Cyperone Alleviates Lung Cell Injury Caused by Staphylococcus aureus via Attenuation of α-Hemolysin Expression
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M. Luo
J. Qiu
Y. Zhang
J. Wang
J. Dong
H. Li
B. Leng
Q. Zhang
X. Niu
S. Zhao
X. Deng
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Abstract
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In this study, we aimed to evaluate the effect of α- cyperone on S. aureus. We used a hemolysin test to examine the hemolytic activity in supernatants of S. aureus cultured with increasing concentrations of α- cyperone. In addition, we evaluated the production of α- hemolysin (Hla) by Western blotting. Real-time RT-PCR was performed to test the expression of hla (the gene encoding Hla) and agr (accessory gene regulator). Furthermore, we investigated the protective effect of α- cyperone on Hla-induced injury of A549 lung cells by live/ dead and cytotoxicity assays. We showed that in the presence of subinhibitory concentrations of α-cyperone, Hla production was markedly inhibited. Moreover, α- cyperone protected lung cells from Hla-induced injury. These findings indicate that α-cyperone is a promising inhibitor of Hla production by S. aureus and protects lung cells from this bacterium. Thus, α-cyperone may provide the basis for a new strategy to combat S. aureus pneumonia.
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KEYWORD
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Staphylococcus aureus, α-cyperone, α-haemolysin, anti-virulence
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